Figure 11.

In vitro CD8⁺ T cell priming using unfractionated PBMC. Dendritic cell differentiation from healthy HLA-A2+ donor PBMCs (n = 7) was induced with GM-CSF (100 ng/ml) and IL-4 (20 ng/ml) and Melan A peptide (1O μg/ml) with M04 (50 μM) or 2,3' cGAMP (5 μg/ml) was added the next day when indicated. On day 11, the primed Metan-A specific CD8⁺ T lymphocytes were detected using tetramer staining within the CD3⁺ T cell population after aggregates and dead cell exclusion. The graph represents the fold increase of Metan-A - specific CD8⁺ T lymphocytes frequency compared to the condition without STING agonists. A non-parametric Friedman signed rank test followed by Dunn's multiple comparison test was used to assess significance. *p < 0.05.