Table 1.

Effect of plant and microbial ligands in vitro on ASICs.

Compound	Trend	Value and object of action	References
(-)-Epigallocatechin gallate	↓	IC50 13.2 μM for mASIC3 in CHO cells	Yan et al., 2019
5-caffeoylquinic acid	↓	IC50 0.235 μM for rASIC in DRG neurons	Qu et al., 2014
Chloroquine	↓	IC50 615.9 μM for rASIC1a in CHO cells	Li et al., 2014
	↑	EC50 425.2 μM for sustained current rASIC3in CHO cells	Lei et al., 2016
Daurisoline	∩	EC50 20 μM for transient current rASIC1a in oocytes	Osmakov et al., 2019b
	↔	EC50 140 μM for rASIC1a in oocytes
Gastrodin	↓	IC50 0.2 μM for rASIC in DRG neurons	Qiu et al., 2014
Ligustrazine	↓	IC50 270 μM for rASIC in DRG neurons	Zhang et al., 2015
	↓	IC50 97 μM for rASIC1a, 62 μM rASIC1b, 129.4 μM rASIC2a, 239.5 μM rASIC3 in CHO cells	Zhang et al., 2015
Lindoldhamine	↓	IC50 9 μM for rASIC1a oocytes (at pH 6.85 stimulus)	Osmakov et al., 2019c
	Δ	EC50 1.53 mM for hASIC3, 3.2 mM rASIC3 in oocytes	Osmakov et al., 2018
	↑	EC50 of 3.8 μM for transient current hASIC3 in oocytes
Morphine	↓	IC50 2.3 μM for rASIC in DRG neurons	Cai et al., 2014
Neomycin	↓	IC50 45 μM for rASIC in DRG neurons	Garza et al., 2010
Paeoniflorin	↓	IC50 5 μM for rASIC in pheochromocytoma cells	Sun et al., 2011
Puerarin	↓	IC50 38.4 μM for rASIC in hippocampal cells, 9.31 μM for rASIC1a in CHO cells	Gu et al., 2010
Quercetin	↓	IC50 2.4 μM for rASIC1a, 1.3 μM for rASIC2a, 1.8 μM for rASIC3 in CHO cells	Mukhopadhyay et al, 2017
Salicylic acid	↓	IC50 260 μM for rASIC3 in COS cells	Voilley et al., 2001
Sevanol	↓	IC50 353 μM for transient hASIC3, 234 μM for sustained hASIC3 currents in oocytes	Dubinnyi et al., 2012
Sinomenine	↓	IC50 0.3 μM for rASIC1a in CHO cells	Wu et al., 2011
Streptomycin	↓	IC50 30 μM for rASIC in DRG neurons	Garza et al., 2010
Thalassiolin B	↓	IC50 27 μM for rASIC in DRG neurons	Garateix et al., 2011

↓, Inhibition of current; ↑, Potentiating of current; Δ, Activation of current without acidic drop; ∩, Generation of 2nd current component; ↔, Inhibition of steady-state desensitization; IC₅₀, half-maximal inhibitory concentration; EC₅₀, half-maximal effective concentration; hASIC, human ASIC; rASIC, rat ASIC; mASIC, mouse ASIC.