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Direct effect of peptic-tryptic digests of gliadin on intestinal enterocytes. Representative images of organ culture of healthy (n = 5) and coeliac (n = 5) biopsies in the presence or absence of peptic-tryptic (PT) digests of gliadin demonstrates direct effects of gliadin. Treatment of coeliac biopsies for 24 h with PT gliadin reveals significant changes in cytokeratin and tubulin staining, as demonstrated by fluorescence microscopy.
