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. 2020 Jun 24;21:492–511. doi: 10.1016/j.omtn.2020.06.015

Figure 1.

Figure 1

Sal-miR-58 May Enter the Mouse Body and Induces VSMC Autophagy and Inhibits the Inflammatory Response in a Mouse AAA Model

(A) The strategy and result analysis of high-throughput second-generation deep sequencing of Salvia miltiorrhiza-derived miRNAs. (B) qRT-PCR analysis of four miRNAs in Salvia miltiorrhiza and mouse VSMCs. ∗∗∗p < 0.001 versus VSMCs (n = 3). (C) qRT-PCR detected the expression of Salvia miltiorrhiza-derived miRNAs in different mouse tissues after Salvia miltiorrhiza was orally administered to ApoE−/− mice for 6 h. ∗∗∗p < 0.001 versus ppt-miR-414 (n = 3 in each group). (D) Sal-miR-58 was determined by qRT-PCR in the serum of mouse AAA models after exogenous administration of Sal-miR-58 for 28 days. ∗∗∗p < 0.001 versus ApoE−/− (n = 5 in each group). (E) miRNAs isolated from mouse serum were treated with/without sodium periodate, and Sal-miR-58 was detected by qRT-PCR. (F) Aortic ultrasonography was used to detect the diameter of abdominal aortas. The data represent the mean ± SEM. ∗∗∗p < 0.001 versus ApoE−/−; ###p < 0.001 versus ApoE−/−+Ang II (n = 5 in each group). (G) Representative photographs of ApoE−/− mouse abdominal aortic dilation after exogenous administration of Sal-miR-58 for 28 days. The data represent the mean ± SEM. ∗∗∗p < 0.001 versus ApoE−/−; ###p < 0.001 versus ApoE−/−+Ang II (n = 5 in each group). (H) Western blot analysis of the inflammatory factors and autophagy markers in mouse AAA tissues. (I) Levels of IL-1β, IL-6, and TNF-α in the serum of mouse AAA models were determined by the ELISA. ∗∗∗p < 0.001 versus ApoE−/−; ###p < 0.001 versus ApoE−/−+Ang II (n = 5 in each group). (J) Immunofluorescence staining of α-SMA (SMA; green), Beclin1 (red) and the nucleus (DAPI; blue) in the injured aortas of control and ApoE−/− mice exposed to Ang II for 28 days. Scale bars, 100 μm. (K) Immunofluorescence staining of α-SMA (SMA; green), LC3B (red) and the nucleus (DAPI; blue) in the injured aortas of control and ApoE−/− mice exposed to Ang II for 28 days. Scale bars, 100 μm.