FIG 9.

MLV sensitivity to mSERINC5 restriction is independent of the route of virus entry. (A and B) NIH 3T3, Mus dunni, and XC cells were infected with F-MLV WT (A) and F-MLV/Ampho^Env (B) (MOI 0.5) in the presence or absence of 30 mM ammonium chloride (NH₄Cl). Cells were harvested 19 h postinfection, and MLV DNA levels were measured by RT-qPCR and normalized to GAPDH. The percentage (%) of relative infectivity was determined with respect to the untreated control (U.T.). (C to E) XC cells were infected with equal amounts of F-MLV WT and gGag^mutF-MLV (C), F-MLV/Ampho^Env (D), and gGag^mutF-MLV/Ampho^Env (E) produced in the presence of mSERINC5 or empty vector. Cells were harvested 5 h postinfection, and MLV DNA levels were measured by RT-qPCR and normalized to GAPDH. The percentage (%) of relative infectivity was determined with respect to virus generated in the presence of empty vector. All results are presented as means ± SD. Statistical significance was determined by unpaired (two-tailed) t test. ***, P < 0.001; ****, P < 0.0001; ns, not significant. Results are shown for n = 3 independent experiments. NH₄Cl, ammonium chloride; U.T., untreated; mS5, mouse SERINC5; E.V., empty vector.