Aflatoxin production of all clone-corrected aflatoxigenic Aspergillus flavus isolates from three populations sampled across the United States, A (n = 21), B (n = 13), and C (n = 1), and from S-strain isolates (n = 2). Isolates from population A produced significantly more aflatoxin than those from population B (P = 0.0038). We did not compare the levels of production of aflatoxin from other groups, as sample sizes were small. Error bars represent the standard errors (SE).