Figure 3. CIV assembly factors co‐migrate with an atypical COX1 submodule that is present in control and COX2Δ cybrids.

• A
  Excerpts of complexome profiling data showing the distribution of CIV subunits and assembly factors HIGD1A, HIGD2A, CMC1, and COA3 in control (143B), COX1Δ, and COX2Δ cybrids. The relative abundance and distribution of detected proteins are shown in the lower graphs. The localization of SC I+III_2plus in COX2Δ cybrids is highlighted with a red line. The approximate molecular weights of CIV‐containing structures are indicated on the top (in kDa).
• B
  2D‐BN/SDS–PAGE analysis from control (143B) and COX2Δ cybrids followed by immunoblotting with the indicated antibodies. I+III₂+IV₁, SC containing CI, CIII, and CIV; I+III_2plus, SC containing CI, CIII, and a COX1 submodule; III₂+IV, SC containing CIII and CIV; IV₂, CIV dimer; IV, CIV monomer. The presence of a COX1‐CMC1‐COA3‐containing subcomplex is indicated.

Data information: See also Fig EV3.Source data are available online for this figure.