Figure 7. Stabilized turnover rate of SC I+III₂ in COX2Δ cybrids.

Mitochondrial translation products were pulse‐labeled in control 143B cells (left panel), and COX1Δ (middle panel) and COX2Δ (right panel) cybrids with [³⁵S]‐methionine for 30 min in the presence of anisomycin to inhibit cytosolic translation. For chase, cells were washed and incubated with fresh culture medium for the indicated time points (T0–T24 h). Radiolabeled mitochondrial proteins (indicated on the right) were separated by 2D‐BN/SDS–PAGE and visualized by autoradiography. An enlarged section of the SC region at T24 (delimited by a square) shows the co‐migration of COX1 with SC I+III_2plus (black arrow) in the COX2Δ cybrids relative to the position of canonical SC I+III₂ (gray arrow). The identities of MRC complexes and SCs (bottom) are as in Fig 1. Molecular weight markers are indicated on the left.

Data information: See also Fig EV5.Source data are available online for this figure.