Figure 3. LifeAct recognizes the closed D‐loop state of F‐actin.

• A, B
  Overlay of F‐actin:ADP‐bound LifeAct (actin in blue and magenta ribbon representation and lifeAct in red cartoon) and jasplakinolide (in gray).
• C
  Representative TIRF images of lifeAct‐binding experiments as indicated. The remaining lifeAct concentration images are shown in Fig EV3. Scale bar = 5 μm.
• D
  Mean ratio of lifeAct fluorescent intensity bound to phalloidin and jasplakinolide F‐actin (mean and SEM; n = 2 or three independent experiments with > 50 actin filament for each set).

Source data are available online for this figure.