Table 1.
Data collection, refinement and validation statistics
| Sample/Parameters | F‐actin–Phalloidin | F‐actin–Apo | F‐actin–Utrophin | F‐actin–LifeAct |
|---|---|---|---|---|
| Microscope | Titan Krios G3 ‐X‐FEG, 300 | |||
| Voltage | 300 | |||
| Defocus range (μ) | −1.5 to −3.0 | −1.5 to −3.0 | −1.8 to −3.3 | −1.8 to −3.5 |
| Camera | Falcon III | Falcon III | Falcon III | Falcon III |
| Pixel size (Å) | 1.38 | 1.08 | 1.38 | 1.38 |
| Total electron dose (e/Å2) | 55.67 | 49.20 | 42.67 | 49.20 |
| Exposure time | 1.99 | 1.99 | 1.99 | 1.99 |
| Frames per movie | 20 | 30 | 20 | 30 |
| Number of images | 1,124 | 529 | 765 | 929 |
| 3‐D refinement statistics and helical symmetry | ||||
| Total number of helical segments extracted | 349,839 | 111,074 | 259,938 | 297,584 |
| Number of segments in map | 91,245 | 64,194 | 149,660 | 74,000 |
| Resolution (Å) | 3.6 | 3.8 | 3.6 | 4.2 |
| Helical twist | −167.02 | −166.8 | −167.34 | −166.9 |
| Rise | 27.89 | 27.25 | 28.02 | 27.44 |
| Map sharpening factor (Å2) | −167.2 | −179.7 | −177.3 | −263 |
| Model composition and validation | ||||
| Non‐hydrogen atoms | 14,722 | 14,421 | 17,394 | 14,733 |
| Protein residues | 1,843 | 1,826 | 2,204 | 1,889 |
| Ligands | 5Mg, 5ADP, 3 Phalloidin | 5Mg, 5ADP | 5Mg, 5ADP | 5Mg, 5ADP |
| RMSD | ||||
| Bond lengths(Å) | 0.012 | 0.008 | 0.006 | 0.009 |
| Bond angles (°) | 0.969 | 0.929 | 0.767 | 0.916 |
| B‐factor (Å2) | ||||
| Protein | 58.50 | 51.76 | 68.95 | 79.6 |
| Actin | 58.2 | 51.5 | 56.1 | 78.2 |
| Toxin/proteina | 51.6 | – | 132.6 | 110.3 |
| Ligand (ADP) | 52.12 | 47.01 | 60.48 | 84.64 |
| MolProbity Score | 2.99 | 2.83 | 2.29 | 3.33 |
| Clashscore | 10.87 | 7.76 | 5.4 | 20.84 |
| Ramachandran plot:‐Favored | 89.98 | 91.00 | 95.38 | 88.10 |
| Allowed | 9.79 | 8.89 | 4.52 | 11.90 |
| Outlier | 0.2 | 0.1 | 0.0 | 0.0 |
| PDB ID | 7BTI | 7BT7 | 6M5G | 7BTE |
| EMDB Code | 30179 | 30171 | 30085 | 300177 |
a
Denotes Phalloidin, Utrophin, and LifeAct in the respective column.