Figure EV3. The ATP synthase‐mediated glycolytic and lipogenic reprogramming (related to Figs 2 and 3).

• A
  Representative images of wt and Low_OXPHOS mice.
• B
  Representative WB expression of Skm proteins related to glycolysis. LDHA levels are significantly augmented in Low_OXPHOS mice when compared to wt. Three samples per condition are shown. Each sample contains extracts from 3 mice. Histograms show the LDHA expression (wt, n = 6; Low_OXPHOS, n = 6).
• C
  ATP levels in Skm from wt and Low_OXPHOS animals (wt, n = 5; Low_OXPHOS, n = 5). No differences were observed.
• D
  Skm free glycerol levels (wt, n = 8; Low_OXPHOS, n = 8).
• E, F
  LD formation upon ATP synthase inhibition (ATPIF1_H49K expression or treatment with 5 μM oligomycin) in C₂C₁₂ cells after 24 h of palmitate supplementation. Blue: DAPI, nuclei; green: BODIPY‐positive LDs. Images are representative of n = 3 experiments, 7–10 fields/condition.
• G
  Representative WB of Skm proteins related with autophagy and inflammation. Two samples per condition are shown. Each sample contains extracts from 3 mice (wt, n = 6; Low_OXPHOS, n = 6).
• H
  Representative WB of proteins related with autophagy in C₂C₁₂ myocytes expressing or not ATPIF1_H49K in the presence or absence of BCAA. No difference where observed. Two samples per condition are shown. n = 3 experiments, 6 replicates/condition.
• I
  Blood glucose levels after 60 days of doxycycline administration in wt (black bars) and Low_OXPHOS (orange bars) mice. No differences were detected between the two genotypes. (wt, n = 12; Low_OXPHOS, n = 12).
• J
  Insulin (ITT) and glucose (GTT) tolerance tests at day 60 of chow diet (wt, n = 12; Low_OXPHOS, n = 12).
• K
  Upper panel. Open field representative track plots of wt and Low_OXPHOS mice when treated or not with edaravone and fed with HFD. No differences were observed. Lower panel. Open field results. Total distance (m), mean speed (m/s), max. speed (m/s), time mobile (s), relative time in the center zone (%) and relative time in the corners (%) of wt (black bars, n = 4), wt + edaravone (light green, n = 4), Low_OXPHOS (orange, n = 4) and Low_OXPHOS + edaravone (dark green, n = 4) are shown.

Data information: Bars are the mean ± SEM of indicated (n) mice. *P < 0.05 when compared to wt by Student's t‐test.Source data are available online for this figure.