Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 4;104(7):999–1004. doi: 10.1136/bjophthalmol-2019-314466

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Expression of Krt10, Krt1 and Krt14 in MGs of the two young donors, one with normal-appearing MGs (36F) and the other with evidence of ductal obstruction (30M). (A, B) Krt10 was expressed in the suprabasal layers of ductules and central duct in 36F (A, red arrows). In the central duct of 30M, the Krt10-positive suprabasal layers (inset in B, red arrow) was ‘sandwiched’ between two Krt10-negative layers, presumably the basal layer (black arrow) and the superficial layer (open arrow). Ductal blockage (arrowhead in B) was caused by Krt10-negative proteins mixed with cell nuclear fragments. (C, D) Krt1 expression level in the MGs of 36F and 30M was high in the ductules and reduced in the suprabasal layer of the central duct (as indicated by arrows in C). Similar to the Krt10 pattern seen in MGs of 30M (B), the superficial layer of the central duct in this donor was negative for Krt1 (open arrows in D). (E, F) Krt14 was highly expressed in the MG acinar and ductule epithelia in both 36F (E) and 30M (F). Krt14 expression was limited to the basal epithelium in the central duct in 36F (arrows in E). The thickness of the Krt14-positive layer was notably increased in the central duct of 30M (F, black arrow in the inset). Scale bar denotes 100 µm. 36F, 36-year-old female; 30M, 30-year-old male; a, acinus; Cd, central duct; d, ductule; MG, meibomian gland.