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. 2020 Jun 2;39(14):e104036. doi: 10.15252/embj.2019104036

Figure 1. ATR inhibition significantly potentiates ionizing radiation (IR)‐induced inflammatory signals.

Figure 1

  1. Hierarchical clustering analysis of RNA sequencing data from cells treated with DMSO, 20 Gy IR, 250 nM AZD6738, or AZD6738 + IR (n = 2 biological replicates).
  2. Top enriched pathway analysis of differentially expressed genes in each cluster as shown in A.
  3. Heat map of 33 representative inflammatory genes regulated by AZD6738 + IR.
  4. MCF10A WT cells were pretreated with DMSO or AZD6738 (250 nM) for 2 h, and then irradiated with 20 Gy, cells were harvested at indicated time points, and immunoblotting was performed with indicated antibodies. h, hours; pSTAT1, pSTAT1(Y701); pIRF3, pIRF3(S396); pTBK1, pTBK1(S172).
  5. MCF10A WT cells were pretreated with DMSO or AZD6738 (250 nM) for 2 h, and then irradiated with indicated dose. 72 h later, cells were harvested for immunoblotting with indicated antibodies.
  6. Same condition as E except the irradiation dose with 20 Gy, mRNA levels of inflammatory genes as indicated were assessed by real‐time quantitative PCR, presented as mean ± standard error of the mean of three biological replicates.