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. 2020 Jun 2;39(14):e104036. doi: 10.15252/embj.2019104036

Figure 4. The MAVS‐dependent cytosolic RNA‐sensing pathway is indispensable for interferon (IFN) signaling induced by ATR inhibition + ionizing radiation (IR).

Figure 4

  • A–F
    (A, C, D, and F) MCF10A WT or two independent clones for MAVS, RIG‐I, MDA5, and IRF7 KO cells were pretreated with DMSO or AZD6738 (250 nM) for 2 h, and then irradiated with 20 Gy; 3 days later, cells were harvested for immunoblotting with indicated antibodies. MAVS, RIG‐I, MDA5, and IRF7 KO were verified by immunoblotting and Sanger DNA sequencing (Appendix Fig S7). (B and E) Same condition as described in A, except that cells were harvested for real‐time quantitative PCR instead of immunoblotting. IFNB1 mRNA level was measured by real‐time quantitative PCR. Data are presented as mean ± standard error of the mean of three biological replicates.

Source data are available online for this figure.