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. 2020 Jun 2;39(14):e104036. doi: 10.15252/embj.2019104036

Figure 6. DNA damage‐elicited AT‐rich DNA mediates type I interferon (IFN) signaling in human cells.

Figure 6

  1. MCF10A WT, cGAS KO, STING KO, and MAVS KO cells were treated with poly(dA‐dT)/LyoVec at indicated concentrations for 24 h. Immunoblotting was performed with indicated antibodies.
  2. 4T1 WT, Sting KO, Mavs KO, and Irf3 KO cells were treated with poly(dA‐dT)/LyoVec at indicated concentrations for 24 h. Immunoblotting was performed with indicated antibodies.
  3. END‐seq was used to map double‐strand breaks (DSBs) associated with TA dinucleotide repeats in MCF10A cells with the indicated treatments. ATR inhibitor (ATRi) AZD6738 (250 nM) was used with ionizing radiation (IR, 10 Gy) for 6 h. NT, not treated.
  4. MCF10A WT, MDA5 KO, RIG‐I KO, and MAVS KO cells were transfected with AT‐rich oligos (2 μg/ml) with LyoVec for 24 h. Immunoblotting was performed with indicated antibodies.
  5. MCF10A WT and STING KO cells were transfected with AT‐rich and non‐AT‐rich oligos (2 μg/ml) with lipofectamine 3000 for 4 h. Immunoblotting was performed with indicated antibodies.
  6. 4T1 WT, Sting KO, and Irf3 KO cells were transfected with AT‐rich and non‐AT‐rich oligos (2 μg/ml) with LyoVec for 24 h. Immunoblotting was performed with indicated antibodies.
  7. A proposed potential mechanism for the divergent cytosolic nucleic acid‐sensing pathway activated in response to radiation alone or with ATRi among different cell lines.