TABLE 1.
Incubation of a SARS‐CoV‐2 contaminated swab with 104 copies/mL sequentially into 10 sample tubes with lysis buffer
| ORF region | E‐gene | |
|---|---|---|
| Tube 1 | 30.37 | 29.91 |
| Tube 2 | 30.59 | 30.29 |
| Tube 3 | 30.6 | 30.26 |
| Tube 4 | 31.32 | 30.95 |
| Tube 5 | 32.52 | 32.36 |
| Tube 6 | 32.19 | 32.01 |
| Tube 7 | 32.84 | 32.83 |
| Tube 8 | 32.61 | 32.68 |
| Tube 9 | 32.21 | 32.01 |
| Tube 10 | 32.1 | 32.14 |
Note: The difference in Ct values between Tube 1 and Tube 10 was 1.73 and 2.23 for the ORF 1a region and the E‐gene, respectively. The experiment was done with Roche media kits. Each tube contains 4.3 mL Roche media buffer.