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. 2020 Jun 8;9(2):14. doi: 10.3390/ht9020014

Table 3.

Evaluation of throughput between traditional and high-throughput luminescence-based SBA (L-SBA).

Method L-SBA Traditional CFU-Based SBA
Total time of execution 7 h 1 1.5 working day 2
Data acquisition 2 min/SBA plate 2–3 h/SBA plate 2
Reproducibility Higher operator independency Lower operator independency
Assay throughput 1 operator/day: 132 individual sera in single (12 SBA plates total) 1 operator/1.5 day: 12 individual sera in single (1 SBA plate 2)

1 To execute one set of 12 SBA plates; 2 to execute one SBA plate, plating each reaction well in one full agar plate: one SBA plate corresponds to 96 agar plates. CFU = Colony Forming Unit.