Table 3.

Kinetic data for the formation of the Michaelis complex with PKA Cα wt in the presence of 0.2 mM AMP-PNP and 1 mM MgCl₂. Data derived from SPR analyses (Figure S5) of the interaction between PKA Cα and GST-PKS or GST-PKT, respectively.

Phosphoryl Acceptor	ka (M−1s−1)	kd (s−1)	KD (nM)
GST-PKS	2.7 × 106	0.89 × 10−2	3.3
GST-PKT	2.2 × 106	29 × 10−2	136