Table 4.

Kinetic data for the formation of the Michaelis complex with AMP-PCP and MnCl₂. Data derived from SPR analyses of the interaction between PKA Cα wt or F187V (Figure S9D,E) and GST-PKS or GST-PKT, respectively, in the presence of 0.2 mM AMP-PCP and 1 mM MnCl₂.

PKA Cα	Phosphoryl Acceptor	ka (M−1s−1)	kd (s−1)	KD (nM)
wt	GST-PKS	2.8 × 106	3.0 × 10−2	11
	GST-PKT	1.7 × 106	41 × 10−2	241
F187V	GST-PKS	1.1 × 106	7.8 × 10−2	71
	GST-PKT	1.7 × 106	4.2 × 10−2	26