Figure 2.

3C12C conjugation with monomethyl auristatin E (3C12C‐MMAE) kill mantle cell lymphoma (MCL) lines in vitro. (a) CD83⁺ KM‐H2, Mino, Rec‐1 or CD83⁻ Z138, and Jvm2 cells were cultured with different concentrations of 3C12C‐MMAE for 72 h before determining viable cells by CellTiter‐Glo Luminescent Cell Viability assay. Data are from one representative experiment (left), and the mean ± SEM of half‐maximal inhibitory concentration (IC50) from three experiments (right) is shown. (b) Mino cells were cultured in 3C12C‐MMAE (antibody concentration of 0.2 µg mL⁻¹) for 18 h, and then, the cells were fixed for cell cycle analysis by flow cytometry. Data are from one representative experiment (upper panel), and the mean ± SEM of three experiments (bottom panel) is shown.