Figure 2.

In vitro characterization and cytocompatibility of the hydrogels. (a) Rheological assessment of HApN loaded with Rif, showing a lower critical solution temperature (LCST) of 26 °C. (b) Cumulative release of rifampicin from 1 cm² polypropylene mesh fragments coated with 50 µL of the Rif-HApN hydrogel (n = 3) and incubated at 37 °C in phosphate buffered saline (PBS). (c) Rabbit fibroblasts (n = 6) exhibited high viability after 24 h of culture under exposure to the HApN and Rif-HApN hydrogels. For each group, symbols depict the samples tested and grey lines represent the median and interquartile range. (d) Flow cytometry scatter dot plots of the different cell cultures evaluated. For each plot, the x-axis represents the forward scatter cell size (FSC-A) and the y-axis represents the propidium iodide fluorescent signal (PerCP-Vio700-A).