Figure S1. sgRNA design for the generation of USP30 KO cell lines and sequence of the two SHSY5Y USP30 KO clones used in this study.

KOD and KOC were generated using sgRNA#1 (target region shown in orange) and KO11 was generated using sgRNA#2 (target region shown in blue). Insertions and deletions are indicated in red. The green codon corresponds to catalytic C77. Frequency of allele detection: KOD allele 1 (7/10), allele 2 (3/10); KOC allele 1 (5/10), allele 2 (5/10); KO11 allele 1 (4/5), allele 2 (1/5). TSS, transcriptional start site.