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. 2020 Jul 7;3(8):e202000768. doi: 10.26508/lsa.202000768

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© 2020 Rusilowicz-Jones et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S2. — (A) SHSY5Y and two independent USP30 KO clones (KOC and KOD) were treated for 4 and 8 h with antimycin A and oligomycin A (AO; 1 μM each) or DMSO, lysed, and analysed by Western blotting. (B, C) Graphs show quantification of ubiquitylated species of MFN2 and TOM20 normalised to the unmodified counterparts. (D) SHSY5Y cells and two USP30 KO clones (KOD and KO11) were treated for 1 and 4 h with AO (1 μM). Cells were homogenised and mitochondrial fractions (MF) prepared and analysed as indicated. (E, F) Bar charts show quantification of (E) ubiquitylated TOM20 normalised to unmodified TOM20 and (F) PINK1. Black and red arrowheads indicate unmodified and ubiquitylated MFN2, TOM20, and FIS1 respectively (high exp, higher exposure).

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