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. 2020 Jul 14;22:14. doi: 10.1186/s12575-020-00123-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Molecular validation of the generated flies. Molecular confirmation of the genomic mutations used to generate aNup107^D364N and btin^mDPE transgenic flies. RFLP screening was used to rapidly detect the relevant strains to be further screened. Final strains were validated using Sanger sequencing, as compared to WT flies, for both cNup107^D364N and dtin^mDPE