Table 2.

Sequences for ssODN design. PAM is underlined, gRNA DSB site is indicated by a slash (/), and the introduced mutations locations are indicated in BOLD

	Nup107	tin
Locus	Chr2L (-)	Chr3R (+)
Genomic sequence	GCCGATTCCAAGAACTATGACGAGTACAGCCGCGCGACGGCGGGTGTCTTCTCCGGCCACTTGGGCTCGCTGAAAACCCTTTTGCACAGCAACTGGCACGATTTGCTCTGGGCCCATC/TGAAGGTGCAGATCGACATCCGTGTGGAATCGGAGATACGCGGCTGCTGCCTCAAAAACTACCAACCGATGCCCGATGATT	TGAGGCGGTCTGGGGTGAAAGGAGTACGCGTTCAGTACCAAAATCGAGCTGACAAATTGCAG/ACACGGTCGAGTGCGCATCGGAACGGCGCTTTACGGCTTACGGGTTACGGACTACTGATTGCCGATTACGGGC
gRNA target	TTTGCTCTGGGCCCATCTGAAGG	GAGCTGACAAATTGCAGACACGG
Designed ssODN	GCCGATTCCAAGAACTATGACGAGTACAGCCGCGCGACGGCGGGTGTCTTCTCCGGCCACTTGGGCTCGCTGAAAACCCTTTTGCACAGCAACTGGCACaATTTGCTCTGGGCtCAcCTGAAGGTGCAGATCGACATCCGTGTGGAATCGGAGATACGCGGCTGCTGCCTCAAAAACTACCAACCGATGCCCGATGATT	TGAGGCGGTCTGGGGTGAAAGGAGTACGCGTTCAGTACCAAAATCGAGCTGACAAATTGCctcatgtGTCGAGTGCGCATCGGAACGGCGCTTTACGGCTTACGGGTTACGGACTACTGATTGCCG