Skip to main content
. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Methods Enzymol. 2019 Oct 22;631:43–59. doi: 10.1016/bs.mie.2019.08.012

Fig. 1.

Fig. 1

Principle of Treg suppression assay. CFSE-labeled Teff are cultured alone, or with an equal amount of CTV-labeled Teff or Tregs and stimulated for 4 days with 1 μg/mL plate-bound anti-CD3 (clone SP34) and 0.1 μg/mL soluble anti-CD28 (clone CD28.2). Cultures are then analyzed for proliferation by FACS (A) and the indicated cytokines are quantified in culture supernatants by Luminex-based bead multiplex immunoassay (B). (A) Representative bivariate FACS plots (CFSE (FITC) vs CTV (Pacific Blue)) of live single CD4+ T cells in each culture condition are reported.