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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Methods Enzymol. 2019 Oct 22;631:43–59. doi: 10.1016/bs.mie.2019.08.012

Fig. 2.

Fig. 2

Immunomagnetic purification of human Tregs. Representative example of Treg and Teff purification from healthy donor derived PBMC using CD4+CD25+ Human Regulatory T Cell Isolation Kit (Miltenyi). (A) FACS plots gated on live single cells showing expression of CD25 and CD4 before (left) and after Tregs (right, top) and Teff (right, bottom) purification. (B) Intracellular staining of Foxp3 in purified Teff and Tregs. FACS plots showing Foxp3 and CD25 expression in CD4+ T cells from Teff and Treg purified fractions.