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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Methods Enzymol. 2019 Oct 22;631:43–59. doi: 10.1016/bs.mie.2019.08.012

Table 1.

Example of FACS antibody panel for assay acquisition.

Channel Target Dilution
FITC CFSE –
PE GITR 1:50
PE-TexasRed CD45RA 1:50
PerCP-Cy5.5 PD-1 1:50
PE-Cy7 CD62L 1:50
Pacific Blue CTV –
AmCyan Viability 1:500
AlexaFluor700 CD4 1:50
APC-Cy7 CD25 1:50

Highlighted in gray are the non-lineage/non-viability markers that need to be substituted with fluorochrome matched isotype controls in the isotype control samples described in Section 4.