Table 1.
Findings on cerebrospinal fluid and serum during the course of the disease.
| Cerebro Spinal Fluid | |||
|---|---|---|---|
| Investigations | |||
| Feb-25 | March-02 | April-03 | |
| Total cells count (n.v. < 4 cells /uL) | 76 | 25 | 16 |
| Proteins (n.v. 20−50 mg/dl) | slightly elevated | 48 | 105 |
| Glucose (n.v.40−80 mg/dl) | normal | 68 | 87 |
| Neurotropic viruses, bacteria and yeast PCR* | neg | neg | neg |
| SARS-Cov-2 PCR | – | – | neg |
| IgG Link index (n.v. < 0.70) | – | 0.67 | 1.45 |
| Isoelectrofocusing IgG | – | Mirror pattern + oligo-clonal bands | none |
| Autoimmune encephalitis and onconeurals Ab# | – | NMDA-R Ab + | NMDA-R Ab + |
| IL6 (pg/ml) ° | – | 4.58 | 5.75 |
| IL8 (pg/ml) ° | – | 40.1 | 744 |
| IL-1ß (pg/ml) ° | – | 0.314 | 0.287 |
| TNF-α (pg/ml) ° | – | 2.17 | 3.24 |
| Serum | |||
| Feb-25 | March-02 | April-03 | |
| Autoimmune encephalitis and onconeurals Ab# | – | neg | neg |
| IL6 (n.v. 0–10 pg/ml) | – | 52 | 206 |
CMV, EBV, HSV1, HSV2, HSV6, HSV7, HSV8, VZV, Adenovirus, Parvovirus, Influenza virus A and B, VRS, Enterovirus, Parechovirus,West Nile virus, Tuscany virus, K pneumoniae, E. coli, B. burgdoferi, T. pallidum, T gondii, L monocitogenes, S. agalactiae, S. pneumoniae, N meningitidis, H influenzae, Criptococcus neoformans and gattii.
NMDA-R, GABA-B R, AMPA-R 1–2, LGI1, CASPR2, DPPX, Amphiphysin, CV2, PNMA2, Ri, Yo, Hu, Recoverin, SOX1, Titin, Zic4, GAD65, Tr Ab.
IL-8, IL-6, Il-1ß and TNF-α were measured by commercially available multiplex bead immunoassays: ELLA Simple Plex ELISA, immunoassays, multianalyte platform (Bio-Techne - Abingdon UK). Bold indicates increased values respect with internal controls data. Cytokines control data were obtained from CSF samples from 12 subjects with functional neurologic disorders.