Table 1.

Antibodies used in the study

Antigen	Source and type of antibody	Immunogen	Specificity Controls	Dilution
ßIII-tubulin	BioLegend Cat# 801212 Mouse monoclonal clone TUJ-1 RRID: AB_2721321	Rat brain microtubules	Validated by immunoblot (Moody etal., 1996). Shows expected pattern of mouse hair cell labeling, as defined (e.g., Stone and Rubel,2000).	1:200
Myosin VIIa	Proteus Biosci. Cat# 25-6790 Rabbit polyclonal RRID: AB_10015251	Amino acids 877-1075 of human myosin VIIa	Validated by immunoblot (Hasson etal., 1995). Shows expected pattern of mouse hair cell labeling, as defined (e.g., Hasson et al., 1997).	1:500
Neurofilament (200 kD)	Millipore Cat# AB5539 Chicken polyclonal RRID: AB_177520	Purified bovine heavyweight neurofilament	Validated by immunoblot (manufacturer). Shows expected pattern of labeling of mouse inner ear nerve fibers, as defined by Del Rio et al. (2013).	1:1000
Neurofilament (~160 kD)	Develop. Studies Hybridoma Bank Mouse monoclonal Cat #2H3 RRID: AB_531793	Embryonic rat spinal cord membrane preparations	Shows expected pattern of labeling of mouse inner ear nerve fibers, as defined by Del Rio et al. (2013).	2 μg/ml
Sox2	Santa Cruz Bioch. Cat# 17320 Goat polyclonal RRID: AB_2286684	Peptides from human Sox2 Cterminus	Validated by immunoblot (manufacturer). Shows expected pattern of mouse hair cell labeling, as defined by Oesterle et al. (2008).	1:100