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. Author manuscript; available in PMC: 2021 Jul 6.
Published in final edited form as: Dev Cell. 2020 Jun 11;54(1):21–32.e5. doi: 10.1016/j.devcel.2020.05.021

Figure 2. Xi Polycomb recruitment can be initiated, but not maintained, without Xist Repeat B.

Figure 2.

See also Figure S2.

(A) H2AK119ub and H3K27me3 IF combined with Xist RNA FISH in WT Xist and ΔRepB differentiating female ESCs.

(B) Quantification of (A). Note that no Xist clouds are present at day 0 to indicate Xmus, but cells accordingly show no focal enrichment of H2AK119ub or H3K27me3. n > 100 per time point.

(C) Allele-specific H2AK119ub/H3K27me3 ChIP-seq timecourse in WT Xist and ΔRepB differentiating female ESCs. Note the 2-fold difference in y-axis scaling between WT Xist and ΔRepB tracks.

(D) Zoom-in of allele-specific ChIP-seq tracks in (C).

(E) Boxplots quantifying allele-specific ChIP-seq coverage over X-linked genes. Wilcoxon rank-sum test, p values as shown.