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. Author manuscript; available in PMC: 2021 Jul 6.
Published in final edited form as: Dev Cell. 2020 Jun 11;54(1):21–32.e5. doi: 10.1016/j.devcel.2020.05.021

Figure 4. Repeat B, but not A, continues to play a role in maintaining Polycomb across Xi.

Figure 4.

See also Figures S1 and S4.

(A) H2AK119ub and H3K27me3 IF combined with Xist RNA FISH in WT Xist, ΔRepA, and ΔRepB female MEFs. n > 100 per deletion cell line, with nearly all cells showing the indicated pattern of enrichment for each mark.

(B) Allele-specific H2AK119ub/H3K27me3 ChIP-seq in WT Xist, ΔRepA, and ΔRepB MEFs.

(C) Zoom-in of allele-specific ChIP-seq tracks in (B).

(D) Boxplots quantifying allele-specific ChIP-seq coverage over X-linked genes. Wilcoxon rank-sum test, p values as shown.

(E) Diagram summarizing roles of Repeats A and B in gene silencing and Polycomb recruitment throughout XCI. Repeat A is required for initial gene silencing and an early wave of Polycomb recruitment; Repeat B is required for sustained gene silencing and Polycomb recruitment. Dashed line indicates uncertain or minimal contribution.