TABLE 1.
Standard cellular morphological parameters utilized for manual and automated cell finding by the CytoCypher system.
| Manual acquisition criteria | |
| 1. Rod shaped morphology with clear and well-organized sarcomere striations throughout the cell body. Cell bodies with artifacts or abnormal bulges were avoided. | |
| 2. Regular contractions upon electrical stimulation. Exclude myocytes with extra or escaped beats | |
| Automatic acquisition criteria | |
| Area | |
| Maximum area (μm2) | 6000 |
| Minimum area (μm2) | 1000 |
| Aspect ratio | |
| Maximum ratio | 0.5 |
| Minimum ratio | 0.1 |
| Misc. | |
| Cell Overlap Parameter (μm; i.e., avoids two cells together) | 40 |
| Number of cells | 50 |
| Well scanning | Spiral motion |
| Operations on full field | Static cell finding with autofocus |