TABLE 3.

Baseline measurements of calcium in myocytes from mouse, rat, and guinea pig using Fura-4f.

			Simple			Hierarchical
	Prep.	n	F/F0	TTP90	TTB90	F/F0	TTP90	TTB90	Suit.	p values
Rat	6	505	1.136 ± 0.003	0.017 ± 0.0002	0.193 ± 0.004	1.134 ± 0.013	0.017 ± 0.0004	0.198 ± 0.019	YYY	Rat vs. Mouse
											F/F0	TTP90	TTB90
										Simple	0.157	<0.0001	<0.0001
										Hierarchical	0.802	<0.0001	<0.0001
Mouse	7	311	1.143 ± 0.004	0.021 ± 0.0004	0.411 ± 0.009	1.131 ± 0.014	0.022 ± 0.0005	0.418 ± 0.017	–	Mouse vs. Guinea Pig
											F/F0	TTP90	TTB90
										Simple	<0.0001	<0.0001	<0.0001
										Hierarchical	–	–	–
Guinea Pig	1	75	1.196 ± 0.001	0.083 ± 0.006	0.564 ± 0.016	–	–	–	–	Guinea Pig vs. Rat
											F/F0	TTP90	TTB90
										Simple	<0.0001	<0.0001	<0.0001
										Hierarchical	–	–	–

Comparison of the mean and standard error of parameters of calcium handling measured by Fura-4f dye labeling of rat, mouse, and guinea pig cardiomyocytes. Advanced statistical tests are applied to mouse and rat populations to indicate statistically significant differences between the kinetic parameters. Results from a number of preparations are presented using simple statistics (mean ± standard deviation) and using hierarchical statistics [mean ± standard deviation and (ICC)]. The p values column presents the calculations of p using Student’s t test or the hierarchical method.