Table 3:
Steady-state kinetic parameters measuring oxygen consumption with L-cysteine analogs.a
| wild-type CDO | C93E CDO | |||||
|---|---|---|---|---|---|---|
|
kcat (s−1) |
Km (mM) |
kcat/Km (M−1s−1) |
kcat (s−1) |
Km (mM) |
kcat/Km (M−1s−1) |
|
| D-cysteine | 0.25 ± 0.02 | 0.2 ± 0.09 | 1200 ± 600 | 0.20 ± 0.03 | 0.7 ± 0.4 | 300 ± 200 |
| cysteamine | 1.1 ± 0.1 | 1.1 ± 0.3 | 1000±300 | 1.2 ± 0.2 | 1.6 ± 0.6 | 800 ± 300 |
| 3-mercaptopropionate | –b | – | – | – | – | – |
a
Kinetic parameters adjusted for iron content.
b
There was no measurable activity under the described experimental conditions.