Table 1. Primers used in the study.
Target |
Primer |
Reference |
|
---|---|---|---|
Forward | Reverse | ||
IFN-α | 5′- GCTGAATGACCTGGAAGCCTGTG -3′ | 5′- GGGAGGTTGTCAGAGCAGAAATC-3′ | Verma et al., 2012 [14] |
IFN-β | 5′-AAGGAGGACGCCG CATTGAC-3′ | 5′-ATAGACATTAGCCAGGAGGTTC-3′ | Self-designed |
IFN-γ | 5′- TCGTTTTGGGTTCTCTTGGC-3′ | 5′-TCCGCTACATCTGAATGACC-3′ | Self-designed |
IRF5 | 5′-CAGGACGGAGATAACACCAT-3′ | 5′-GGTGTATTTCCCTGTCTCCT-3′ | Self-designed |
IRF7 | 5′-AAAACCAACTTCCGCTGC-3′ | 5′-GCCTCAGTCTGGTCCGTGC-3′ | Self-designed |
β-actin | 5′- CGGCATCGTCACCAACTGG-3′ | 5′- ACGTTGCTATCCAGGCTGTGC-3′ | Verma et al., 2012 [14] |
IFI44 | 5′-GAGAGATGTGAGCCTGTGAGG-3′ | 5′-TTTTCCTTGTGCACAGTTGAT-3′ | Self-designed |
FCγR1 | 5′-CTT CTC CTT CTA TGT GGG CAG T-3′ | 5’-GCT ACC TCG CAC CAG TAT GAT-3′ | Zhang et al., 2014 [15] |