Fig 3. Nucleotide substitutions accumulate with age.

Higher median mutation frequencies were observed in brain, muscle, single oocytes, and oocyte pools of mothers compared with those of pups. Permutation test p-values (one-sided test based on medians, corrected for multiple testing) are shown with the respective sample sizes (n). In the calculation of mutation frequencies for single oocytes, the numbers of mutations and sequenced nucleotides were combined across all oocytes analyzed for a mouse. Considering only samples sequenced at a DCS depth ≥100× does not change the results qualitatively (S5B Fig). The raw data for the information depicted in this figure are available at https://github.com/makovalab-psu/mouse-duplexSeq. DCS, duplex consensus sequence.