Table 1. Features of cloned and functionally tested monoclonal antibodies.
Monoclonal antibodies derived from iDCgB immunized mice (DC, n = 4), HCMV infected mice (CV, n = 2), and iDCgB immunized and protected against HCMV (n = 3). Control antibodies SM5-1 and KIOVIG are shown as references. CB unit = cord blood units used for humanization. EC50 binding capacity to recombinant gB protein (μg/ml) was calculated for clones DC06, DC14 and DC16, PR28 and PR32. IC50 HCMV-neutralizing capacity (μg/ml) was calculated for clones DC06, DC16 and PR32. V, D and J genes depict top matches according to IgBLAST and IMGT nomenclature. Amino acid (AA) mutations include substitutions, insertions, and deletions. Characterictics of heavy chains are depicted.
| AB # | COHORT | SUBJECT | CB unit | EC50 gB binding (μg/ml) | IC50 NT (μg/ml) | VH_GENE | DH_GENE | JH_GENE | VH_IDENTITY | CDRH3_AA | CDRH3_AA_LENGTH | CH_ISOTYPE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CV03 | HCMV-Control | Mouse-473 | 346 | - | - | 3–30*18,3-30-5*01 | 3–10*02 | 6*03 | 98,3 | AKPVRGVYYSYYMDV | 15 | 3–1 |
| CV04 | HCMV-Control | Mouse-475 | 346 | - | - | 3–30*18,3-30-5*01 | 3–10*01,3–10*02,6–6*01 | 6*03 | 97,3 | AKPPRSGYYYYYMDV | 15 | 3–1 |
| PR32 | iDCgB-HCMV | Mouse-1113 | 313 | 0.74 | 0,58 | 3–74*01 | 6–6*01 | 4*02 | 98,6 | QCRSIADRRSPEFDY | 15 | 2–1 |
| PR35 | iDCgB-HCMV | Mouse-499 | 346 | 0.05 | - | 3–48*02 | 5–18*01,5–5*01 | 6*03 | 90,5 | ARVTYSYGSPHQYYCMDV | 18 | 1–1 |
| PR28 | iDCgB-HCMV | Mouse-1113 | 313 | - | - | 4–4*02 | 3–10*01 | 5*02 | 94,9 | ARKPKVMVRGISDFFDP | 17 | 1–1 |
| DC06 | iDCgB | Mouse-1157 | 184 | 0.29 | 0,09 | 3–30*18,3-30-5*01 | 7–27*01 | 3*02 | 95,2 | AKPFASGWGWVNAFDI | 16 | 1–1 |
| DC14 | iDCgB | Mouse-1254 | 229 | 0.004 | - | 1–46*01 | 2–2*01,2–2*03 | 4*02 | 98,3 | ARGLRVYCSSSTCYATAGDY | 20 | 2–1 |
| DC16 | iDCgB | Mouse-1254 | 229 | 0.66 | 15,94 | 3–7*03 | 3–22*01 | 3*02 | 90,5 | VRDRNYHEGSTYYDVFDI | 18 | 1–1 |
| DC17 | iDCgB | Mouse-1255 | 229 | - | - | 1–46*01 | 4–17*01 | 4*02 | 91,6 | ARDHGDYHFFDS | 12 | 1–1 |
| SM5-1 | Reference | - | - | 0,09 | 0,014 | 1–2*02 | 2–8*01 | 3*01 | 91,9 | ARDGAKTVSNSGLSLLYYHNRLDA | 14 | - |
| Kiovig | Reference | - | - | 0,6 | 9,8 | - | - | - | - | - | - | - |