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. 2020 Jul 15;15(7):e0236119. doi: 10.1371/journal.pone.0236119

Fig 4. Effects of disulfiram and sunitinib on apoptosis and detection of caspase-3/7 activation in RCC cell lines.

Fig 4

(A) NPL4 protein expression levels in RCC cell lines treated with control, disulfiram, sunitinib, or the combination of disulfiram and sunitinib for 48 h were determined by western blotting analyses. (B) Apoptosis assays were performed using flow cytometry at 72 h after drug treatment (*, P < 0.0001). (C) Caspase-3/7 activity was measured by determining fluorescence intensity in RCC cells treated with control, disulfiram, sunitinib, or the combination of disulfiram and sunitinib for 48 h (*, P < 0.001; **, P < 0.0001; left). Representative images of caspase-3/7 activity in RCC cell lines (right).