Figure 5.

Effects of RIG-I and MDA5 KO by CRISPR-Cas9 on SFTSV-triggered antiviral and inflammatory responses. A, WB analysis of RIG-I- or MDA5-KO HEK293 cells with or without SFTSV infection. Two cell clones with RIG-I or MDA5 KO were respectively generated by CRISPR/Cas9-mediated gene editing and infected with SFTSV or mock-infected. At 24 hpi, the indicated protein expression was monitored by WB. Data are representative of two independent experiments with similar results. B and C, effects of RIG-I or MDA5 KO on SFTSV-stimulated cytokine expression. RIG-I- or MDA5-KO cells or the control cells were infected with SFTSV or mock-infected. At 24 hpi, relative mRNA levels (B) and secreted protein levels (C) of the indicated cytokines were analyzed by qRT-PCR and ELISA, respectively. Data are presented as means ± S.D. (error bars), n = 3. ***, p < 0.001.