Table 3.
List of primers for real-time quantitative PCR
| Primer namea | Primer sequence (5′–3′) |
|---|---|
| RIG-I-F | GCCTTCAGACATGGGACGAA |
| RIG-I-R | ACTGCTTTGGCTTGGGATGT |
| MDA5-F | TGTGCTGGACTACCTGACCT |
| MDA5-R | ACGAATTCCCGAGTCCAACC |
| MAVS-F | AGCTAGTTGATCTCGCGGAC |
| MAVS-R | TTCTCTCCTGGGGACTCTGG |
| IFN-β-F | CACTACAGCTCTTTCCATGA |
| IFN-β-R | AGCCAGTGCTAGATGAATCT |
| TNF-α-F | AAAACAACCCTCAGACGCCA |
| TNF-α-R | GCTACAGGCTTGTCACTCGG |
| MxA-F | GCTACACACCGTGACGGATATGG |
| MxA-R | CGAGCTGGATTGGAAAGCCC |
| OAS2-F | CGGTGTATGCCTGGGAACAGG |
| OAS2-R | GGGTCAACTGGATCCAAGATTAC |
| ISG56-F | CCTCCTTGGGTTCGTCTACA |
| ISG56-R | GGCTGATATCTGGGTGCCTA |
| ISG15-F | TCCTGGTGAGGAATAACAAGGG |
| ISG15-R | GTCAGCCAGAACAGGTCGTC |
| IL-8-F | GGACAAGAGCCAGGAAGAAACC |
| IL-8-R | TCTCAGCCCTCTTCAAAAACTTCT |
| RANTES-F | GGCAGCCCTCGCTGTCATCC |
| RANTES-R | GCAGCAGGGTGTGGTGTCCG |
| IP-10-F | AGGAACCTCCAGTCTCAGCA |
| IP-10-R | CAAAATTGGCTTGCAGGAAT |
| SFTSV S segment-F | ACATTTTCCCTGATGCCTTG |
| SFTSV S segment-R | GCTGAAGGAGACAGGTGGAG |
| SFTSV M segment-F | TGTGGAGGGATGCGTGTCAGA |
| SFTSV M segment-R | AGTGGAATTGAATCTCCGTGCT |
| SFTSV L segment-F | TCACGCCACTGCTTTCGCTTT |
| SFTSV L segment-R | CGGCTCCTGACAATGTTCCT |
| TRIM25-F | CGAGGTGGAACTGAACCACA |
| TRIM25-R | GGACAGGGGGAGGTTTCTTG |
| GAPDH-F | ACCACAGTCCATGCCATCAC |
| GAPDH-R | TCCACCACCCTGTTGCTGTA |
aF, forward primer; R, reverse primer.