Figure 3.

An OLE RNA fragment containing P13 and flanking regions is sufficient to form a complex with OapB. A, characteristics of OapB binding to the OLE-2 RNA construct, which lacks the P14.1 region. Shaded nucleotides identify a UNCG tetraloop that was used in place of a portion of P14 and all of P14.1. OapB concentrations range from 1 pm to 1 μm (half-log increments). Additional annotations are as described in the legend to Fig. 2. B, OapB fails to bind the OLE-3 RNA construct, which lacks the P13 region. OapB concentrations range from 320 pm to 1 μm (half-log increments). Additional annotations are as described in the legend to Fig. 2.