Figure 11.

Thermal stability by DSC, overlay of melting curves. A, an overlay of anti–TAA-1 BEAT bsAbs with and without D84.4Q, as well as the corresponding homodimers. The first transition corresponds to the melting of both the CH2 and CH3 domains, as the peaks overlap. B, an overlay of anti–TAA-1 BEAT (B) half-antibodies with and without the D84.4Q mutation. The corresponding homodimers are also shown for comparison. Because of the low Cp values of the transitions for the Fc region, a magnification of the boxed area is shown. C, an overlay of anti–TAA-1 KiH bsAb and the corresponding homodimers. D, anti–TAA-1 KiH (H) half-antibody overlaid with the corresponding homodimer. Each curve shows a transition at ∼90 °C that corresponds to the melting of the Fab portion.