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. 2020 May 13;295(28):9392–9408. doi: 10.1074/jbc.RA119.012335

Figure 4.

Figure 4.

Thermal stability by DSC showing that D84.4Q has no impact on stability in the heterodimeric context. A, an overlay of BEAT Q3A-D84.4Q Fc (solid line) and BEAT Fc (dashed line) is shown. The first was built with an IgG3 CH2 domain in the BEAT (A) chain, whereas the latter had an IgG1 CH2 domain. The peak corresponds to the melting transitions (Tm) of both the CH2 and CH3 domain as the transitions overlap. The Tm values were ∼69.0 and ∼70.0 °C, respectively. B, similar to before but BEAT Q3A-D84.4Q Fc (solid line) encompassed an IgG1 CH2 domain in the BEAT (A) chain, whereas BEAT Fc (dashed line) had an IgG3 CH2 domain. The Tm values were ∼70.0 and ∼69.0 °C, respectively.