Table 2.
Summary of calorimetric enthalpies (ΔHcal) and Tm values of Fc regions. The table presents a summary of calorimetric enthalpies (ΔHcal) and Tm values observed for the Fc regions of anti–TAA-1 BEAT D84.4Q, BEAT, and KiH. The data obtained for homodimers, heterodimers, and half-antibodies are shown
| Construct | ΔHcal ± S.E. | Tm |
|---|---|---|
| kcal/mol | °C | |
| Anti–TAA-1 BEAT (B) D84.4Q half-antibody | 5.8 ± 0.6 | 47.3 and 54.9 |
| Anti–TAA-1 (VH2) BEAT (B) D84.4Q half-antibody | 5.1 ± 0.5 | 47.1 and 55.1 |
| Anti–TAA-1 BEAT (B) half-antibody | 8.2 ± 1.5 | 54.2 |
| Anti–TAA-1 KiH (H) half-antibody | 10.4 ± 0.4 | 58.2 |
| Anti–TAA-1 BEAT (A) homodimer | 8.3 ± 0.6 | 65.3 |
| Anti–TAA-1 BEAT (B) D84.4Q homodimer | 6.7 ± 0.8 | 54.3 |
| Anti–TAA-1 BEAT (B) homodimer | 13.2 ± 0.7 | 58.7 |
| Anti–TAA-1 KiH (K) homodimer | 20.9 ± 0.7 | 60.9 |
| Anti–TAA-1 KiH (H) homodimer | 20.5 ± 0.5 | 60.6 |
| Anti–TAA-1 BEAT D84.4Q heterodimer | 27.0 ± 0.7 | 69.7 |
| Anti–TAA-1 BEAT heterodimer | 26.9 ± 0.7 | 69.3 |
| Anti–TAA-1 KiH heterodimer | 24.0 ± 0.6 | 71.0 |