Figure 2.
NleD orthologues cleave JNK and p38 but not ERK. A, An NleD orthologue phylogenetic tree was created with Clustal Omega. Proteins selected for further analysis are highlighted in boldface. B, NleD orthologues of C. rodentium (NleDCR1 and NleDCR2), S. enterica serovar Arizonae (NleDSEA), and H. defensa (NleDHD) were coexpressed in E. coli BL21 together with hexahistidine-tagged MAP kinases (i.e. p38β, JNK2, or ERK1). Proteins were then extracted from the bacteria, and MAPK cleavage was probed by Western blot analysis using the anti-6His antibody. Intact and fragmented MAPKs are indicated by blue and red arrowheads, respectively.