Table 2.
Summary of NleD proteolysis results
| MAP kinase substrate | Result for NleDEPEC |
|
|---|---|---|
| In vitro | In vivo | |
| Wild type | ||
| ERK | No | No |
| p38 | Yes | Yes |
| JNK | Yes | Yes |
| Activation loop | ||
| JNK P184E | Yes | |
| ERKA-p38 | Yes | |
| ERKA-JNK | Yes | |
| p38A-ERK | Yes | |
| GST-JNK2169-192-GFP | Yes | |
| GST-JNK2176-192-GFP | Yes | |
| GST-JNK2180-192-GFP | Yes | |
| GST-JNK2176-188-GFP | Yes | |
| GST-JNK2180-186-GFP | Yes | |
| GST-JNK2182-188-GFP | Yes | |
| GST-ERKA-GFP | Yes | |
| G-helix | ||
| ERΚG-p38 | No | |
| ERKG-JNK | No | |
| MKI | ||
| ERKm-p38 | No | |
| ERKm-JNK | No | |
| G-helix + MKI + L16 | ||
| ERKG,M,l-p38 | No | No |
| ERKG,M,l-JNK | No | No |
| αEF/αF loop | ||
| ERΚF-p38 | No | |
| G-helix + αEF/αF loop | ||
| ERKG,F-p38 | No | |
| G-helix + MKI + L16 + αEF/αF loop | ||
| ERKG,F,M,l-p38 | No | |