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. 2020 May 14;295(28):9502–9512. doi: 10.1074/jbc.RA120.013690

Figure 1.

Figure 1.

STEAP1 purification and stability. A, UV-visible spectrum of the purified STEAP1-Fab120.545 complex used for EM sample preparation. The protein and heme absorbance peaks exhibit maxima at 280 and 412 nm, respectively. B, size-exclusion chromatography elution profile of STEAP1 in the absence (red) or presence (blue) of excess Fab120.545. Fractions collected from the STEAP1-Fab120.545 sample were used for EM sample preparation. C, SDS-PAGE of the purified STEAP1-Fab120.545 complex. D, heme absorbance size-exclusion chromatography elution profiles of STEAP1 after 10 min of incubation at several temperatures. E, melting curve for digitonin-purified STEAP1, generated by using the peak maxima from D. STEAP1 exhibits a melting temperature of 55.5 °C.