Figure 2.

Cryo-EM structure of the STEAP1-Fab120.545 complex. A, exemplary 2D class averages. The box size corresponds to 300 × 300 pixels (309 × 309 Å). The length of the scale bar in the top right corner is 100 Å. More 2D class averages are shown in Fig. S2B. B, two orientations of the unsharpened cryo-EM density map at 3.0-Å resolution. The STEAP1 chains are colored blue, cyan and green; and the Fab molecules are colored brown, magenta and light-gray. C and D, atomic model of the STEAP1-Fab120.545 complex as viewed parallel to the membrane as a side view (C) or orthogonal to the membrane from the cytoplasmic side (D). In D, the Fabs are shown as surfaces.