Figure 5.

G9a/Glp and Hdac1/2 repress the expression of Pcgf6 targeted germline genes. A, IP–Western blot analysis showing physical interaction of Pcgf6, Hdac1/2, and G9a/Glp in cell extracts from WT and Pcgf6^−/− ESCs. B, Western blot analysis showing the methylation levels of H3K9 in G9a^−/−, Glp^−/−, and G9a/Glp^−/− ESCs. C, Western blot analysis showing the acetylation levels of H3 in Hdac1^−/− and Hdac2^−/− ESCs. D, the mRNA levels of germline-related genes in ESCs of the indicated genotype or in ESCs treated with the indicated inhibitors. All cells were treated with inhibitors for 72 h. DMSO is the solvent control. Error bars, S.D. of three independent experiments. The asterisks arranged vertically refer to significant differences compared with WT ESCs. Horizontally arranged asterisks indicate significant differences between groups.